| First Author | Tian W | Year | 2005 |
| Journal | Cell Immunol | Volume | 235 |
| Issue | 1 | Pages | 72-84 |
| PubMed ID | 16143319 | Mgi Jnum | J:107956 |
| Mgi Id | MGI:3622591 | Doi | 10.1016/j.cellimm.2005.07.004 |
| Citation | Tian W, et al. (2005) Silencing OCILRP2 leads to intrinsic defects in T cells in response to antigenic stimulation. Cell Immunol 235(1):72-84 |
| abstractText | We have previously demonstrated that OCILRP2 interaction with its ligand NKRP1f provides a co-stimulatory signal for optimal T cell proliferation and IL-2 production. Here, using RNA interference technology, we will demonstrate that silencing OCILRP2 in vivo leads to intrinsic impairment in T cell response to CD3- and CD28-cross-linking as well as antigenic stimulation. OCILRP2-silenced T cells have reduced cell proliferation and IL-2 production, which can be bypassed by PMA and ionomycin treatment. OCILRP2-silenced T cells also failed to undergo TCR capping and had impaired cytoskeleton reorganization. Moreover, in OCILRP2-silenced T cells, tyrosine phosphorylation of Lck was diminished, while tyrosine phosphorylation of linkers for activation of T cells was unchanged. Interestingly, NF-kappaB activation was also impaired as the result of OCILRP2 silencing. Together, our data strongly support a novel role for OCILRP2 C-type lectin in TCR-mediated signal transduction. The observation that OCILRP2 is involved in TCR capping and cytoskeletal organization suggests that OCILRP2-NKRP1f may facilitate lipid rafts and immunological synapse formation during T cell interaction with antigen presenting cells. |
