| First Author | Hosseinpour F | Year | 2007 |
| Journal | FEBS Lett | Volume | 581 |
| Issue | 25 | Pages | 4937-42 |
| PubMed ID | 17904126 | Mgi Jnum | J:126925 |
| Mgi Id | MGI:3762312 | Doi | 10.1016/j.febslet.2007.09.024 |
| Citation | Hosseinpour F, et al. (2007) Overexpression of the Rho-guanine nucleotide exchange factor ECT2 inhibits nuclear translocation of nuclear receptor CAR in the mouse liver. FEBS Lett 581(25):4937-42 |
| abstractText | Various drugs such as phenobarbital (PB) trigger translocation of constitutive active/adrostane receptor (CAR) from the cytoplasm into the nucleus of mouse liver cells without directly binding to the receptor. We have now characterized the guanine nucleotide exchange factor epithelial cell-transforming gene 2 (ECT2) as a PB-inducible factor as well as a cellular signal that represses PB-triggered nuclear translocation of CAR. When CFP-tagged ECT2 was co-expressed with YFP-tagged CAR in the liver of Car(-/-) mice, ECT2 repressed CAR nuclear translocation. Coexpression of various deletion mutants delineated this repressive activity to the tandem Dbl homology/pleckstrin homology domains of ECT2 and to their cytosolic expression. CAR directly bound to the PH domain. Thus, ECT2 may comprise a part of the PB response signal regulating the intracellular trafficking of CAR. |
