| First Author | Polo-Parada L | Year | 2001 |
| Journal | Neuron | Volume | 32 |
| Issue | 5 | Pages | 815-28 |
| PubMed ID | 11738028 | Mgi Jnum | J:73253 |
| Mgi Id | MGI:2154808 | Doi | 10.1016/s0896-6273(01)00521-9 |
| Citation | Polo-Parada L, et al. (2001) Alterations in Transmission, Vesicle Dynamics, and Transmitter Release Machinery at NCAM-Deficient Neuromuscular Junctions. Neuron 32(5):815-28 |
| abstractText | Although functional neuromuscular junctions (NMJs) form in NCAM-deficient mice, they exhibit multiple alterations in presynaptic organization and function. Profound depression and unusual periodic total transmission failures with repetitive stimulation point to a defect in vesicle mobilization/cycling, and these defects were mimicked in (+/+) NMJs by inhibitors of myosin light chain kinase, known to affect vesicle mobilization. Two separate release mechanisms, utilizing different endocytic machinery and Ca(2+) channels, were shown to coexist in (-/-) terminals, with the mature process targeted to presynaptic membrane opposed to muscle, and an abnormally retained immature process targeted to the remainder of the presynaptic terminal and axon. Thus, NCAM plays a critical and heretofore unsuspected role in the molecular organization of the presynaptic NMJ. |
