| First Author | Bietrix F | Year | 2006 |
| Journal | J Biol Chem | Volume | 281 |
| Issue | 11 | Pages | 7214-9 |
| PubMed ID | 16421100 | Mgi Jnum | J:110557 |
| Mgi Id | MGI:3640629 | Doi | 10.1074/jbc.M508868200 |
| Citation | Bietrix F, et al. (2006) Accelerated lipid absorption in mice overexpressing intestinal SR-BI. J Biol Chem 281(11):7214-9 |
| abstractText | Dietary cholesterol absorption contributes to a large part of the circulating cholesterol. However, the mechanism of sterol intestinal uptake is not clearly elucidated. Scavenger receptor class B type I (SR-BI), major component in the control of cholesterol homeostasis, is expressed in the intestine, but its role in this organ remains unclear. We have generated transgenic mice overexpressing SR-BI primarily in the intestine by using the mouse SR-BI gene under the control of intestinal specific 'apoC-III enhancer coupled with apoA-IV promoter.' We found SR-BI overexpression with respect to the natural protein along the intestine and at the top of the villosities. After a meal containing [(14)C]cholesterol and [(3)H]triolein, SR-BI transgenic mice presented a rise in intestinal absorption of both lipids that was not due to a defect in chylomicron clearance nor to a change in the bile flow or the bile acid content. Nevertheless, SR-BI transgenic mice showed a decrease of total cholesterol but an increase of triglyceride content in plasma without any change in the high density lipoprotein apoA-I level. Thus, we described for the first time a functional role in vivo for SR-BI in cholesterol but also in triglyceride intestinal absorption. |
