| First Author | Sedjaï F | Year | 2010 |
| Journal | J Cell Sci | Volume | 123 |
| Issue | Pt 14 | Pages | 2391-401 |
| PubMed ID | 20551181 | Mgi Jnum | J:172939 |
| Mgi Id | MGI:5009352 | Doi | 10.1242/jcs.065045 |
| Citation | Sedjai F, et al. (2010) Control of ciliogenesis by FOR20, a novel centrosome and pericentriolar satellite protein. J Cell Sci 123(Pt 14):2391-401 |
| abstractText | Cilia and flagella are evolutionary conserved organelles that generate fluid movement and locomotion, and play roles in chemosensation, mechanosensation and intracellular signalling. In complex organisms, cilia are highly diversified, which allows them to perform various functions; however, they retain a 9+0 or 9+2 microtubules structure connected to a basal body. Here, we describe FOR20 (FOP-related protein of 20 kDa), a previously uncharacterized and highly conserved protein that is required for normal formation of a primary cilium. FOR20 is found in PCM1-enriched pericentriolar satellites and centrosomes. FOR20 contains a Lis1-homology domain that promotes self-interaction and is required for its satellite localization. Inhibition of FOR20 expression in RPE1 cells decreases the percentage of ciliated cells and the length of the cilium on ciliated cells. It also modifies satellite distribution, as judged by PCM1 staining, and displaces PCM1 from a detergent-insoluble to a detergent-soluble fraction. The subcellular distribution of satellites is dependent on both microtubule integrity and molecular motor activities. Our results suggest that FOR20 could be involved in regulating the interaction of PCM1 satellites with microtubules and motors. The role of FOR20 in primary cilium formation could therefore be linked to its function in regulating pericentriolar satellites. A role for FOR20 at the basal body itself is also discussed. |
