| First Author | Boon KL | Year | 2010 |
| Journal | FEBS Lett | Volume | 584 |
| Issue | 15 | Pages | 3299-304 |
| PubMed ID | 20621096 | Mgi Jnum | J:201306 |
| Mgi Id | MGI:5512951 | Doi | 10.1016/j.febslet.2010.07.001 |
| Citation | Boon KL, et al. (2010) Deletion of Swm2p selectively impairs trimethylation of snRNAs by trimethylguanosine synthase (Tgs1p). FEBS Lett 584(15):3299-304 |
| abstractText | The 5' cap trimethylation of small nuclear (snRNAs) and several nucleolar RNAs (snoRNAs) by trimethylguanosine synthase 1 (Tgs1p) is required for efficient pre-mRNA splicing. The previously uncharacterised protein Swm2p interacted with Tgs1p in yeast two-hybrid screens. In the present study we show that Swm2p interacts with the N-terminus of Tgs1p and its deletion impairs pre-mRNA splicing and pre-rRNA processing. The trimethylation of spliceosomal snRNAs and the U3 snoRNA, but not other snoRNAs, was abolished in the absence of Swm2p, indicating that Swm2p is required for a substrate-specific activity of Tgs1p. |
