Primary Identifier | MGI:6436272 | Allele Type | Transgenic |
Attribute String | Reporter | Gene | Tg(Kit-EGFP)3Poz |
Strain of Origin | (C57BL/6 x DBA)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The transgene construct consists of an enhanced GFP (EGFP) gene carrying at its 5' an HA epitope followed by a nuclear localization signal and, at its 3' end, stop codons, and a polyA addition site, was used as a reporter. The coding sequence of the EGFP gene was fused, at its initial ATG, to the first ATG of the first exon (and 6.7-kb 5 flanking sequence) of the Kit gene. Construct 3 used in this line also included a 4.5-kb XbaI-BamHI fragment of the first intron. |