| First Author | Decalf J | Year | 2014 |
| Journal | PLoS Pathog | Volume | 10 |
| Issue | 7 | Pages | e1004269 |
| PubMed ID | 25079788 | Mgi Jnum | J:248529 |
| Mgi Id | MGI:5919675 | Doi | 10.1371/journal.ppat.1004269 |
| Citation | Decalf J, et al. (2014) Establishment of murine gammaherpesvirus latency in B cells is not a stochastic event. PLoS Pathog 10(7):e1004269 |
| abstractText | Murid gamma-herpesvirus-4 (MuHV-4) promotes polyclonal B cell activation and establishes latency in memory B cells via unclear mechanisms. We aimed at exploring whether B cell receptor specificity plays a role in B cell susceptibility to viral latency and how this is related to B cell activation. We first observed that MuHV-4-specific B cells represent a minority of the latent population, and to better understand the influence of the virus on non-MuHV-4 specific B cells we used the SWHEL mouse model, which produce hen egg lysozyme (HEL)-specific B cells. By tracking HEL+ and HEL- B cells, we showed that in vivo latency was restricted to HEL- B cells while the two populations were equally sensitive to the virus in vitro. Moreover, MuHV-4 induced two waves of B cell activation. While the first wave was characterized by a general B cell activation, as shown by HEL+ and HEL- B cells expansion and upregulation of CD69 expression, the second wave was restricted to the HEL- population, which acquired germinal center (GC) and plasma cell phenotypes. Antigenic stimulation of HEL+ B cells led to the development of HEL+ GC B cells where latent infection remained undetectable, indicating that MuHV-4 does not benefit from acute B cell responses to establish latency in non-virus specific B cells but relies on other mechanisms of the humoral response. These data support a model in which the establishment of latency in B cells by gamma-herpesviruses is not stochastic in terms of BCR specificity and is tightly linked to the formation of GCs. |
