| Primary Identifier | MGI:5705514 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout, Reporter | Gene | Mrln |
| Strain of Origin | (C57BL/6 x C3H)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | TALEN mediated recombination inserted a tdTomato reporter and triple polyadenylation cassette into exon 1. Detection of tdTomato fluorescence was specific to skeletal muscle and was not detected in other tissues, such as tendon. Real-time PCR analysis using primer pairs specific to the downstream exons 2 and 3 confirmed the absence of transcript in skeletal muscle as well as in liver. |
