| First Author | Duong HA | Year | 2014 |
| Journal | Nat Struct Mol Biol | Volume | 21 |
| Issue | 2 | Pages | 126-32 |
| PubMed ID | 24413057 | Mgi Jnum | J:210023 |
| Mgi Id | MGI:5569417 | Doi | 10.1038/nsmb.2746 |
| Citation | Duong HA, et al. (2014) Temporal orchestration of repressive chromatin modifiers by circadian clock Period complexes. Nat Struct Mol Biol 21(2):126-32 |
| abstractText | The mammalian circadian clock is built on a molecular feedback loop in which the Period (PER) proteins, acting in a large, poorly understood complex, repress Clock-Bmal1, the transcription factor driving their expression. We found that mouse PER complexes include the histone methyltransferase HP1gamma-Suv39h. PER proteins recruited HP1gamma-Suv39h to the Per1 and Per2 promoters, and HP1gamma-Suv39h proved important for circadian di- and trimethylation of histone H3 Lys9 (H3K9) at the Per1 promoter, feedback repression and clock function. HP1gamma-Suv39h was recruited to the Per1 and Per2 promoters ~4 h after recruitment of HDAC1, a PER-associated protein previously implicated in clock function and H3K9 deacetylation at the Per1 promoter. PER complexes containing HDAC1 or HP1gamma-Suv39h appeared to be physically separable. Circadian clock negative feedback by the PER complex thus involves dynamic, ordered recruitment of repressive chromatin modifiers to DNA-bound Clock-Bmal1. |
