| First Author | Zhu XJ | Year | 2007 |
| Journal | Nat Cell Biol | Volume | 9 |
| Issue | 2 | Pages | 184-92 |
| PubMed ID | 17237772 | Mgi Jnum | J:166017 |
| Mgi Id | MGI:4839446 | Doi | 10.1038/ncb1535 |
| Citation | Zhu XJ, et al. (2007) Myosin X regulates netrin receptors and functions in axonal path-finding. Nat Cell Biol 9(2):184-92 |
| abstractText | Netrins regulate axon path-finding during development, but the underlying mechanisms are not well understood. Here, we provide evidence for the involvement of the unconventional myosin X (Myo X) in netrin-1 function. We find that Myo X interacts with the netrin receptor deleted in colorectal cancer (DCC) and neogenin, a DCC-related protein. Expression of Myo X redistributes DCC to the cell periphery or to the tips of neurites, whereas its silencing prevents DCC distribution in neurites. Moreover, expression of DCC, but not neogenin, stimulates Myo X-mediated formation and elongation of filopodia, suggesting that Myo X function may be differentially regulated by DCC and neogenin. The involvement of Myo X in netrin-1 function was further supported by the effects of inhibiting Myo X function in neurons. Cortical explants derived from mouse embryos expressing a motor-less Myo X exhibit reduced neurite outgrowth in response to netrin-1 and chick commissural neurons expressing the motor-less Myo X, or in which Myo X is silenced using microRNA (miRNA), show impaired axon projection in vivo. Taken together, these results identify a novel role for Myo X in regulating netrin-1 function. |
