| Year | 1981 | Journal | Hereditas |
| Volume | 94 | Issue | 1 |
| Pages | 1-20 | PubMed ID | 6111546 |
| Mgi Jnum | J:14977 | Mgi Id | MGI:63124 |
| Citation | Pfister K, et al. (1981) Abstracts of papers presented at the Workshop on Molecular Genetics of the Mouse II. August 11-16, 1980, Sandbjerg Slot, Sonderborg, Denmark. (A systemic regulatory locus associated with two new structural alleles of murine beta-glucuronidase in M. musculus castaneus. Hereditas 94(1):1-20 (18) |
| abstractText | Full text of Abstract: 29. A systemic regulatory locus associated with two new structural alleles of murine Beta-glucuronidase in M. musculus castaneus. K. PFISTER1, V. CHAPMAN2 and K. PAIGEN2 Department of Metabolism, University Children's Hospital, Zurich, Switzerland1, and Department of Molecular Biology, Roswell Park Memorial Institute, Buffalo, USA2. In two congenic strains, constructed by transferring the structural regulatory gene complex [Gus] of Beta-glucuronidase from M. musculus castaneus onto the C57BL/6 inbred strain background, the structure and the regulation of the expression of Beta- glucuronidase were characterized. Two novel structural forms of Beta-glucuronidase were identified, differing in thermolability and, in one case, in antigenicity from any previously known Beta-glucuronidase allelozyme. They were found to have the same molecular activity as the Beta-glucuronidase from reference strain C57BL/6. The results indicate the presence of new alleles of the structural gene of Beta-glucuronidase. Both mutant structural genes in the congenic strains were similarly regulated by temporal and regulatory gene(s). The development course of the Beta-glucuronidase activity expression paralleled that of reference strain C57BL/6 but remained at approx. half the activity level in all organs studied. In kidney, the androgen-inducible rate of synthesis of Beta-glucuronidase was lowered to approx. half of that in C57BL/6. Thus, the two [Gus] haplotypes of the congenic strains appear to carry mutations in a previously unidentified systemic regulatory locus that appears to alter the rate of Beta-glucuronidase synthesis by a constant factor. It exhibits additive inheritance with F1 Heterozygotes showing intermediate enzyme levels. |
