| First Author | Kogiso M | Year | 1992 |
| Journal | Jpn J Ophthalmol | Volume | 36 |
| Issue | 3 | Pages | 281-90 |
| PubMed ID | 1361208 | Mgi Jnum | J:4990 |
| Mgi Id | MGI:53469 | Citation | Kogiso M, et al. (1992) Endotoxin-induced uveitis in mice. 1. Induction of uveitis and role of T lymphocytes. Jpn J Ophthalmol 36(3):281-90 |
| abstractText | Endotoxin-induced uveitis (EIU) with a high frequency of posterior iris synechiae was induced by the systemic injection of 200 micrograms of endotoxin into C3H/HeN mice, an endotoxin-responsive strain. The cell number and the protein concentration within the aqueous humor began to increase 6 hours after the injection, achieving a peak at 24 hours, and decreased gradually thereafter. Inflammatory cells were observed in the anterior chamber, the vitreous body and near the iris-ciliary body histologically. Most of the inflammatory cells were polymorphonuclear cells. On the other hand, C3H/HeJ mice, an endotoxin-unresponsive strain, showed no increase in either cell number or protein concentration in the aqueous humor after endotoxin administration. Pretreatment of C3H/HeN mice with anti-Thy-1.2 antibody significantly decreased both the cell number and the protein concentration in the aqueous humor and the incidence of the posterior synechiae, as compared with the control group. Anti-CD4 antibody also significantly reduced the severity of EIU, while anti-CD8 antibody had no influence on the disease. Anti-IFN-gamma antibody increased the cell number in the aqueous humor. These observations indicate that T lymphocytes, especially CD4+ T lymphocytes, have an extremely important role in the development of EIU in mice. |
