| First Author | Deshayes S | Year | 2023 |
| Journal | EJNMMI Res | Volume | 13 |
| Issue | 1 | Pages | 103 |
| PubMed ID | 38019303 | Mgi Jnum | J:346531 |
| Mgi Id | MGI:7616576 | Doi | 10.1186/s13550-023-01039-5 |
| Citation | Deshayes S, et al. (2023) [(18)F]FDG PET-MR characterization of aortitis in the IL1rn(-/-) mouse model of giant-cell arteritis. EJNMMI Res 13(1):103 |
| abstractText | BACKGROUND: Metabolic imaging is routinely used to demonstrate aortitis in patients with giant-cell arteritis. We aimed to investigate the preclinical model of aortitis in BALB/c IL1rn(-/-) mice using [(18)F]fluorodeoxyglucose ([(18)F]FDG) positron emission tomography-magnetic resonance (PET-MR), gamma counting and immunostaining. We used 15 first-generation specific and opportunistic pathogen-free (SOPF) 9-week-old IL1rn(-/-) mice, 15 wild-type BALB/cAnN mice and 5 s-generation specific pathogen-free (SPF) 9-week-old IL1rn(-/-). Aortic [(18)F]FDG uptake was assessed as the target-to-background ratio (TBR) using time-of-flight MR angiography as vascular landmarks. RESULTS: [(18)F]FDG uptake measured by PET or gamma counting was similar in the first-generation SOPF IL1rn(-/-) mice and the wild-type group (p > 0.05). However, the first-generation IL1rn(-/-) mice exhibited more interleukin-1beta (p = 0.021)- and interleukin-6 (p = 0.019)-positive cells within the abdominal aorta than the wild-type mice. In addition, the second-generation SPF group exhibited significantly higher TBR (p = 0.0068) than the wild-type mice on the descending thoracic aorta, unlike the first-generation SOPF IL1rn(-/-) mice. CONCLUSIONS: In addition to the involvement of interleukin-1beta and -6 in IL1rn(-/-) mouse aortitis, this study seems to validate [(18)F]FDG PET-MR as a useful tool for noninvasive monitoring of aortitis in this preclinical model. |
