| Primary Identifier | MGI:5780968 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout | Gene | Ctsl |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was replaced with a modified exon 2 in which the conventional start codon M1 ATG was substituted by TTC. A floxed polyadenylation (STOP) sequence was inserted downstream of the modified exon 2. Flp-mediated recombination removed the FRT-flanked puromycin resistance cassette inserted into intron 2. This approach generates a constitutive null allele with the potential to express an M1-mutated cathepsin L mRNA from the endogenous gene locus. |
