| Primary Identifier | MGI:5444035 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Eif4e2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site and FRT flanked neo cassette were inserted upstream of exon 4 and a loxP site was inserted downstream of exon 6 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 4 - 6. Immunoblot analysis confirmed the absence of protein expression in MEFs from homozygous mice. |
