| Primary Identifier | MGI:3838182 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence, RMCE-ready | Gene | Ttr |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | ES cells containing the Ttrtm1Kymm null allele that serves as acceptor in recombination mediated cassette exchange were electroporated with a replacement vector and a cre recombinase expression vector. The replacement vector consisted of a wild-type human TTR cDNA and a FRT-flanked puromycin resistance gene flanked by a lox66 and a loxP site. Northern blot analysis indicated gene expression in the brain, liver and eye of homozygote mice was similar to endogenous expression in wild-type mice. Human but not mouse TTR protein was detected in the liver and serum of homozygote mice in immunoblot assays. |
