| First Author | Kwak EL | Year | 1990 |
| Journal | Gene | Volume | 94 |
| Issue | 2 | Pages | 255-61 |
| PubMed ID | 2258056 | Mgi Jnum | J:28598 |
| Mgi Id | MGI:76120 | Doi | 10.1016/0378-1119(90)90396-9 |
| Citation | Kwak EL, et al. (1990) Murine ferritin heavy chain: isolation and characterization of a functional gene. Gene 94(2):255-61 |
| abstractText | A mouse liver genomic library screened with a full-length cDNA encoding murine ferritin heavy chain (mFHC) [Torti et al., J. Biol. Chem. 263 (1988) 12638-12644] yielded a functional genomic clone mFHC. The genomic clone isolated included a region of approximately 3 kb containing four exons and three introns. Sequence comparisons of the mouse genomic clone with other genomic clones from rat, human and chicken showed a high degree of similarity among species in the coding regions. Introns and flanking sequences were less conserved. However, comparison of mFHC promoter elements with FHC genes from other species revealed common elements. Analysis of the genomic structure of FHC suggested the presence of pseudogenes. S1 nuclease analysis, however, confirmed that this mouse clone, when transfected into human MRC-5 fibroblasts, was transcribed, indicating that this clone contains an FHC functional gene. |
