| First Author | Wei L | Year | 2011 |
| Journal | FASEB J | Volume | 25 |
| Issue | 9 | Pages | 3068-78 |
| PubMed ID | 21646399 | Mgi Jnum | J:175570 |
| Mgi Id | MGI:5286032 | Doi | 10.1096/fj.11-187252 |
| Citation | Wei L, et al. (2011) Mitochondrial superoxide flashes: metabolic biomarkers of skeletal muscle activity and disease. FASEB J 25(9):3068-78 |
| abstractText | Mitochondrial superoxide flashes (mSOFs) are stochastic events of quantal mitochondrial superoxide generation. Here, we used flexor digitorum brevis muscle fibers from transgenic mice with muscle-specific expression of a novel mitochondrial-targeted superoxide biosensor (mt-cpYFP) to characterize mSOF activity in skeletal muscle at rest, following intense activity, and under pathological conditions. Results demonstrate that mSOF activity in muscle depended on electron transport chain and adenine nucleotide translocase functionality, but it was independent of cyclophilin-D-mediated mitochondrial permeability transition pore activity. The diverse spatial dimensions of individual mSOF events were found to reflect a complex underlying morphology of the mitochondrial network, as examined by electron microscopy. Muscle activity regulated mSOF activity in a biphasic manner. Specifically, mSOF frequency was significantly increased following brief tetanic stimulation (18.1+/-1.6 to 22.3+/-2.0 flashes/1000 mum(2).100 s before and after 5 tetani) and markedly decreased (to 7.7+/-1.6 flashes/1000 mum(2).100 s) following prolonged tetanic stimulation (40 tetani). A significant temperature-dependent increase in mSOF frequency (11.9+/-0.8 and 19.8+/-2.6 flashes/1000 mum(2).100 s at 23 degrees C and 37 degrees C) was observed in fibers from RYR1(Y522S/WT) mice, a mouse model of malignant hyperthermia and heat-induced hypermetabolism. Together, these results demonstrate that mSOF activity is a highly sensitive biomarker of mitochondrial respiration and the cellular metabolic state of muscle during physiological activity and pathological oxidative stress.-Wei, L., Salahura, G., Boncompagni, S., Kasischke, K. A., Protasi, F., Sheu, S.-S., Dirksen, R. T. Mitochondrial superoxide flashes: metabolic biomarkers of skeletal muscle activity and disease. |
