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Publication : Identification of four novel ADAMs with potential roles in spermatogenesis and fertilization.

First Author  Zhu GZ Year  1999
Journal  Gene Volume  234
Issue  2 Pages  227-37
PubMed ID  10395895 Mgi Jnum  J:56266
Mgi Id  MGI:1340718 Doi  10.1016/s0378-1119(99)00208-5
Citation  Zhu GZ, et al. (1999) Identification of four novel ADAMs with potential roles in spermatogenesis and fertilization. Gene 234(2):227-37
abstractText  The ADAM (A Disintegrin And Metalloprotease) family is known to have important roles in various developmental systems, e.g., myogenesis and neurogenesis. In this study, we searched for ADAMs that may function in spermatogenesis or fertilization, and have cloned and sequenced four new mouse ADAM cDNAs: ADAM 24, ADAM 25, ADAM 26 and ADAM 27. The deduced amino acid sequences show that all four contain the complete domain organization common to ADAM family members. Messenger RNA for each of the four ADAMs was found only in the testis. The conserved zinc-dependent metalloprotease active site HEXGHXXGXXHD was found in the metalloprotease domain of three of the novel ADAMs, suggesting that they are testis-specific proteases, to which we give the alternative names: testase 1, ADAM 24; testase 2, ADAM 25; and testase 3, ADAM 26. Using RNA extracted from testes of pre-pubertal males of increasing age (8-40days), we found that adult levels of transcription, assessed in Northern blots, are reached by day 20 (ADAM 27), day 25 (ADAMs 24 and 25) and in the range day 25-50 (ADAM 26). These results suggest that each ADAM is transcribed in spermatogenic cells in a regulated pattern at a specific developmental stage.
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