| First Author | Wegner J | Year | 2016 |
| Journal | Matrix Biol | Volume | 56 |
| Pages | 24-41 | PubMed ID | 27234307 |
| Mgi Jnum | J:239435 | Mgi Id | MGI:5828726 |
| Doi | 10.1016/j.matbio.2016.05.001 | Citation | Wegner J, et al. (2016) Laminin alpha5 in the keratinocyte basement membrane is required for epidermal-dermal intercommunication. Matrix Biol 56:24-41 |
| abstractText | Laminin alpha5 is broadly expressed in the epidermal basement membrane (BM) of mature mice and its elimination at this site (Lama5Ker5 mouse) results in hyperproliferation of basal keratinocytes and a delay in hair follicle development, which correlated with upregulation of the dermally-derived laminin alpha2 and laminin alpha4 chains in the epidermal BM and of tenascin-C subjacent to the BM. In vitro studies revealed laminin 511 to be strongly adhesive for primary keratinocytes and that loss of laminin alpha5 does not result in cell autonomous defects in proliferation. Flow cytometry reveals that the loss of laminin alpha5 resulted in increased numbers of CD45+, CD4+ and CD11b+ immune cells in the skin, which temporo-spatial analyses revealed were detectable only subsequent to the loss of laminin alpha5 and the appearance of the hyperproliferative keratinocyte phenotype. These findings indicate that immune cell changes are the consequence and not the cause of keratinocyte hyperproliferation. Loss of laminin alpha5 in the epidermal BM was also associated with changes in the expression of several dermally-derived growth factors involved in keratinocyte proliferation and hair follicle development in adult but not new born Lama5Ker5 skin, including KGF, EGF and KGF-2. In situ binding of FGF-receptor-2alpha (IIIb)-Fc chimera (FGFR2IIIb) to mouse skin sections revealed decoration of several BMs, including the epidermal BM, which was absent in Lama5Ker5 skin. This indicates reduced levels of FGFR2IIIb ligands, which include KGF and KGF-2, in the epidermal BM of adult Lama5Ker5 skin. Our data suggest an initial inhibitory effect of laminin alpha5 on basal keratinocyte proliferation and migration, which is exacerbated by subsequent changes in growth factor expression by epidermal and dermal cells, implicating laminin alpha5 in epidermal-dermal intercommunication. |
