| First Author | Nakauchi H | Year | 1987 |
| Journal | Nucleic Acids Res | Volume | 15 |
| Issue | 10 | Pages | 4337-47 |
| PubMed ID | 3495785 | Mgi Jnum | J:37454 |
| Mgi Id | MGI:84847 | Doi | 10.1093/nar/15.10.4337 |
| Citation | Nakauchi H, et al. (1987) Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2. Nucleic Acids Res 15(10):4337-47 |
| abstractText | We present here the sequence of the 5310 base pair Hind III-cleaved genomic DNA segment that includes the gene for the Lyt-2, a murine differentiation antigen expressed on most immature T lymphocytes as well as the cytotoxic suppressor T cell subset. We also present the complete intron/exon structure of Lyt-2. There are five exons: a fused leader and immunoglobulin variable region like exon, a hinge region exon, a transmembrane exon and two alternatively spliced intracytoplasmic exons (alternative splicing of these exons yields the 38 kDa alpha and 34 kDa alpha' Lyt-2 polypeptides). The promotor region contains a TATA box and sequences homologous to the putative immunoglobulin transcriptional control elements cd/pd. S1 protection analysis reveals that thymocytes, T cells from lymph nodes, and a Lyt-2 transfectant obtained by introduction of total genomic DNA have the same initiation site. In the 3' region, there is a polyadenylation signal sequence after a 700 bp long 3' untranslated region. |
