| First Author | Speirs S | Year | 1988 |
| Journal | Gamete Res | Volume | 21 |
| Issue | 2 | Pages | 179-84 |
| PubMed ID | 3229730 | Mgi Jnum | J:147184 |
| Mgi Id | MGI:3839554 | Doi | 10.1002/mrd.1120210208 |
| Citation | Speirs S, et al. (1988) Effect of exogenous hormones on the ovulation of primary and secondary oocytes in LT/Sv strain mice. Gamete Res 21(2):179-84 |
| abstractText | LT/Sv strain mice ovulate both primary and secondary oocytes. These are fertilizable and give rise to digynic triploid and normal diploid conceptuses, respectively. A previous study [Kaufman and Speirs, 1987] had indicated that just over 20% of embryos recovered on the 10th day of gestation from spontaneously ovulating females had a triploid chromosome constitution. This value was considerably lower than might have been expected by extrapolation from earlier studies in which LT/Sv mice had been given exogenous gonadotrophins. In the present study, therefore, cytogenetic analysis of fertilized eggs was performed at the first cleavage mitosis in (1) spontaneously ovulating females mated to F1 hybrid males, and (2) superovulated females mated to similar males. Additional females from group (1) were autopsied on the 10th day of gestation, and the ploidy of embryos isolated at this stage of gestation was determined. Exposure to exogenous gonadotrophins significantly increased the proportion of eggs that were ovulated as primary oocytes (34.4%), compared to the situation observed following spontaneous ovulation (24.4%). All the triploids encountered in both series were of the digynic type and characteristically (for LT/Sv mice) had an oocyte-derived set with 40 chromosomes present, and a sperm-derived set containing 20 chromosomes. Similar numbers of eggs were recovered from spontaneously ovulating females on the 1st and 10th days of gestation, and the incidence of triploidy observed on the 10th day was 22.1%. The influence of exogenous hormones in increasing the 'spontaneous' level of triploidy in LT/Sv and in other strains of mice is briefly reviewed. |
