Primary Identifier | MGI:7279067 | Allele Type | Targeted |
Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | B6(Cg)-Tyr<c-2J>/J |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector is designed with, from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed cassette containing a green fluorescent protein (GFP) sequence and a polyadenylation signal, a myc-tagged endoplasmic reticulum-directed promiscuous biotin ligase sequence (BirA*G3-ER), an internal ribosome entry site (IRES), and an mKate2 red fluorescent protein sequence. This targeting vector, which also includes an attB-neoR-attP cassette, was inserted into the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neo cassette. |