First Author | Tryfonidou MA | Year | 2011 |
Journal | Dev Dyn | Volume | 240 |
Issue | 3 | Pages | 663-73 |
PubMed ID | 21337464 | Mgi Jnum | J:169056 |
Mgi Id | MGI:4939809 | Doi | 10.1002/dvdy.22569 |
Citation | Tryfonidou MA, et al. (2011) Novel type II collagen reporter mice: New tool for assessing collagen 2alpha1 expression in vivo and in vitro. Dev Dyn 240(3):663-73 |
abstractText | We report the generation of a new mouse strain harboring a Col2-pd2EGFP reporter transgene; pd2EGFP has a much shorter half-life than EGFP, making it a near real-time reporter for Col2alpha1 expression in vivo and in vitro. In the post-natal growth plate, pd2EGFP fluorescence was expressed in almost all proliferative chondrocytes and in some hypertrophic chondrocytes based on localization with type X collagen. In articular cartilage, pd2EGFP fluorescence diminished over time, nicely illustrating the decrease of type II collagen synthesis in articular chondrocytes during growth. Monolayers of FACS-sorted chondrocytes from P1-2 mice showed faster loss of pd2EGFP compared to EGFP, reflecting rapid chondrocyte de-differentiation. High-density culture of FACS-pd2EGFP- growth plate chondrocytes revealed the typical temporal expression pattern in which type II collagen preceded type X collagen matrix deposition. The Col2-pd2EGFP reporter mouse will be a valuable tool for studies of growth plate chondrocyte biology. Developmental Dynamics 240:663-673, 2011. (c) 2011 Wiley-Liss, Inc. |