Primary Identifier | MGI:2667593 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Prkdc |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 6 was disrupted by the insertion of a neomycin selection cassette. Shortened transcipts were detected in homozygous mutant ES cells by RT-PCR analysis, however neither normal or aberrantly sized protein was detected by Western blot analysis. Assays of cell extracts indicated an ablation of endogenous protein activity. |