| First Author | Mancini A | Year | 2000 |
| Journal | FEBS Lett | Volume | 482 |
| Issue | 1-2 | Pages | 54-8 |
| PubMed ID | 11018522 | Mgi Jnum | J:218007 |
| Mgi Id | MGI:5616330 | Doi | 10.1016/s0014-5793(00)02036-6 |
| Citation | Mancini A, et al. (2000) The direct association of the multiple PDZ domain containing proteins (MUPP-1) with the human c-Kit C-terminus is regulated by tyrosine kinase activity. FEBS Lett 482(1-2):54-8 |
| abstractText | We have identified the multiple PDZ domain containing protein (MUPP-1 or MPDZ) as a novel binding partner of the human c-Kit. c-Kit binds specifically to the 10th PDZ domain of MUPP-1 via its C-terminal sequence. Furthermore, a kinase negative-mutant receptor interacted more strongly with MUPP-1 than the wild-type c-Kit. Strikingly, a constitutively activated c-Kit (D816V-Kit) did not bind to MUPP-1, although this oncogenic form retains the PDZ binding motif 'HDDV' at the C-terminal end. Deletion of V967 of c-Kit abolished binding to MUPP-1 and drastically reduced its tyrosine kinase activity, suggesting that the structure of the C-terminal tail of c-Kit influences its enzymatic activity. |
