| First Author | Lynch BA | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 375 |
| Issue | 4 | Pages | 491-5 |
| PubMed ID | 18692481 | Mgi Jnum | J:140322 |
| Mgi Id | MGI:3813376 | Doi | 10.1016/j.bbrc.2008.07.145 |
| Citation | Lynch BA, et al. (2008) Visualization of SV2A conformations in situ by the use of Protein Tomography. Biochem Biophys Res Commun 375(4):491-5 |
| abstractText | The synaptic vesicle protein 2A (SV2A), the brain-binding site of the anti-epileptic drug levetiracetam (LEV), has been characterized by Protein Tomography. We identified two major conformations of SV2A in mouse brain tissue: first, a compact, funnel-structure with a pore-like opening towards the cytoplasm; second, a more open, V-shaped structure with a cleft-like opening towards the intravesicular space. The large differences between these conformations suggest a high degree of flexibility and support a valve-like mechanism consistent with the postulated transporter role of SV2A. These two conformations are represented both in samples treated with LEV, and in saline-treated samples, which indicates that LEV binding does not cause a large-scale conformational change of SV2A, or lock a specific conformational state of the protein. This study provides the first direct structural data on SV2A, and supports a transporter function suggested by sequence homology to MFS class of transporter proteins. |
