| Primary Identifier | MGI:2446436 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Ckmm-Ucp1)1Boud |
| Strain of Origin | (C57BL/6J x DBA/2J)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Two transgenic lines, MCK-UCP1-13 and MCK-UCP1-20, were established. Both lines express the transgene in heart and muscle tissues, with expression levels varying in different muscles that is not consistent between the two lines. |
| molecularNote | The transgene consists of a rat Ucp1 cDNA sequence under the control of a mouse Ckmm promoter, which drove expression in skeletal muscle and heart. An SV40 polyadenylation signal was added to the Ucp1 construct. Transgene expression in heart and muscle tissues was confirmed by Northern blot analysis. |
