| Primary Identifier | IPR045105 | Type | Family |
| Short Name | Med25/PTOV1 |
| description | This entry includes mediator of RNA polymerase II transcription subunit 25 (Med25) and prostate tumor-overexpressed gene 1 protein (PTOV1). PTOV1 shares protein sequence similarity with Med25, however, it has not been identified as a component of the Mediator complex. PTOV1 is required for cell proliferation [].Mediator is a large complex of up to 33 proteins that is conserved from plants to fungi to humans - the number and representation of individual subunits varying with species [, ]. It is arranged into four different sections, a core, a head, a tail and a kinase-active part, and the number of subunits within each of these is what varies with species. Overall, Mediator regulates the transcriptional activity of RNA polymerase II, but it would appear that each of the four different sections has a slightly different function []. The overall function of the full-length Med25 is efficiently to coordinate the transcriptional activation of RAR/RXR (retinoic acid receptor/retinoic X receptor) in higher eukaryotic cells. Human Med25 consists of several domains with different binding properties, the N-terminal, VWA domain, an SD1 - synapsin 1 - domain from residues 229-381, a PTOV(B) or ACID domain from 395-545, an SD2 domain from residues 564-645 and a C-terminal NR box-containing domain (646-650) from 646-747. The PTOV domain is the domain through which Med25 co-operates with the histone acetyltransferase CBP, which is involved in chromatin remodeling []. |
