| First Author | Shirohzu H | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 369 |
| Issue | 3 | Pages | 862-7 |
| PubMed ID | 18325326 | Mgi Jnum | J:134229 |
| Mgi Id | MGI:3785163 | Doi | 10.1016/j.bbrc.2008.02.110 |
| Citation | Shirohzu H, et al. (2008) Methylation of a conserved intronic CpG island of mouse SF-1 is associated with cell-specific expression of SF-1 in a culture system but not with tissue-specific expression. Biochem Biophys Res Commun 369(3):862-7 |
| abstractText | The mechanism for the steroidogenic tissue or cell-specific expression of SF-1 has not been well clarified. We examined whether the methylation status of a large CpG island in the first intron of mouse SF-1 gene is associated with the expression level of SF-1 in cultured cells and in tissues. The island consists of three small islands (ICI-1, ICI-2, and ICI-3). In cultured adrenocortical Y-1 cells and in Leydig tumor cells, I-10, that both express high levels of SF-1, the upstream region of ICI-2, ICI-2-1, was clearly hypomethylated compared to cultured mouse bone marrow cells that do not express SF-1. However, this methylation status was not clearly associated with the tissue-specific expression of SF-1, in either adult or during development. These results suggest that methylation of ICI-2-1of SF-1 may partly determine the level of SF-1 expression at the cellular level, but may not be essential at the tissue level. |
