| First Author | Nakamura N | Year | 2001 |
| Journal | J Neurochem | Volume | 76 |
| Issue | 1 | Pages | 29-38 |
| PubMed ID | 11145975 | Mgi Jnum | J:67774 |
| Mgi Id | MGI:1931386 | Doi | 10.1046/j.1471-4159.2001.00004.x |
| Citation | Nakamura N, et al. (2001) Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development. J Neurochem 76(1):29-38 |
| abstractText | Since most brain glycoproteins from beta-1,4-galactosyltransferase (beta-1,4-GalT) I knockout mice were galactosylated without apparent reduction the gene expression of novel beta-1,4-GalTs II and V which are involved in N-linked oligosaccharide biosynthesis in addition to beta-1,4-GalT I was studied during mouse brain development. Isolation and characterization of beta-1,4-GalT II and V cDNAs from mouse brains indicates that they are also functioning in the brain. Northern blot analysis revealed that the beta-1,4-GalT I gene is expressed mainly in mid-embryonic stages, while the expression level of beta-1,4-GalT II transcript remains constant and of beta-1,4-GalT V transcript increases during mouse brain development after birth. In situ hybridization revealed that beta-1,4-GalT II and V signals are present in most neural cells, with a marked difference between them in the hippocampus of adult mouse brain tissue. The differential gene expression of beta-1,4-GalTs I, II and V during mouse brain development could affect the differential galactosylation of brain glycoproteins, as revealed by lectin blot analysis. |
