| First Author | Lefebvre L | Year | 2001 |
| Journal | Nat Genet | Volume | 27 |
| Issue | 3 | Pages | 257-8 |
| PubMed ID | 11242104 | Mgi Jnum | J:68878 |
| Mgi Id | MGI:1933661 | Doi | 10.1038/85808 |
| Citation | Lefebvre L, et al. (2001) Selection for transgene homozygosity in embryonic stem cells results in extensive loss of heterozygosity. Nat Genet 27(3):257-8 |
| abstractText | Embryonic stem cells offer unprecedented opportunities for random or targeted genome alterations in the mouse. We present here an efficient strategy to create chromosome-specific loss of heterozygosity in embryonic stem cells. The combination of this method with genome-wide mutagenesis in ES cells (using chemical mutagens or gene-trap vectors) opens up the possibility for in vitro or in vivo functional screening of recessive mutations in the mouse. |
