| First Author | Lakshmanan G | Year | 1998 |
| Journal | Dev Biol | Volume | 204 |
| Issue | 2 | Pages | 451-63 |
| PubMed ID | 9882482 | Mgi Jnum | J:51930 |
| Mgi Id | MGI:1327474 | Doi | 10.1006/dbio.1998.8991 |
| Citation | Lakshmanan G, et al. (1998) Partial rescue of GATA-3 by yeast artificial chromosome transgenes. Dev Biol 204(2):451-63 |
| abstractText | GATA-3 is essential for murine embryonic development, but elucidating the genetic controls over the complex temporal and tissue-specific transcriptional regulatory pattern of this transcription factor gene has been problematic. Here we report the isolation and characterization of two yeast artificial chromosomes (YACs) bearing the murine GATA-3 gene. Ordered deletions of both YACs show that they define a 1-megabase pair contig spanning the GATA-3 locus. We found that a 120-kb YAC transgene, including 35 kb of 5' as well as 60 kb of 3' flanking sequence, confers normal GATA-3 expression at sites not revealed previously through analysis of plasmid transgenic lines. However, even this 120-kb YAC does not contain sufficient information to recapitulate the complete GATA-3 expression program during embryogenesis. While not complete in its regulatory capacity, the YAC transgene is nonetheless able to complement several homozygous GATA-3 mutant phenotypes and thereby prolong embryonic life. Copyright 1998 Academic Press. |
