| First Author | Burnside SS | Year | 1984 |
| Journal | Proc Natl Acad Sci U S A | Volume | 81 |
| Issue | 16 | Pages | 5204-8 |
| PubMed ID | 6206494 | Mgi Jnum | J:109274 |
| Mgi Id | MGI:3628630 | Doi | 10.1073/pnas.81.16.5204 |
| Citation | Burnside SS, et al. (1984) A molecular hybrid of the H-2Dd and H-2Ld genes expressed in the dm1 mutant. Proc Natl Acad Sci U S A 81(16):5204-8 |
| abstractText | Sequential immunoprecipitates show that H-2dm1 mutant cells express a hybrid 'H-2D/L' antigen exhibiting determinants normally associated with two different gene products of the parental d haplotype-i.e., the H-2Dd and H-2Ld antigens. The hybrid H-2D/Ldm1 antigen appears to consist of a portion of the NH2-terminal extracellular half of the H-2Dd antigen 'fused' to a portion of the COOH-terminal extracellular half of the H-2Ld antigen. This structure is inferred from the reactivity of dm1 antigens with cytotoxic T lymphocytes specific for H-2Ld determinants and with monoclonal antibodies specific for determinants in the structural domains of H-2Ld or H-2Dd. The H-2D/Ldm1 molecule apparently retains all of the third external domain (C2 or alpha 3) and part of the second external domain (C1 or alpha 2) of H-2Ld, but its first external domain (N or alpha 1) derives from H-2Dd. From these findings and from previous peptide mapping studies, we propose that the H-2D/Ldm1 antigen is the product of a hybrid gene that has resulted from an unequal crossover between the parental H-2Dd and H-2Ld genes, leaving the N exon and part of the C1 exon of the H-2Dd gene joined to the H-2Ld gene beginning somewhere within its C1 exon. |
