Primary Identifier | MGI:5788548 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Hacd1 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The KOMP targeting vector (PRPGS00067_A_B09) introduced an FRTt site, beta-galactosidase cassette, loxP site, neomhycin resistance cassette, FRT site, and loxP site upstream of exon 2 in addition to inserting a loxP site downstream of exon 4. Cre-mediated recombination removed the selection cassette and exons 2 through 4. The beta-galactosidase reporter gene remains intact. |