| First Author | Jansa P | Year | 1996 |
| Journal | Nucleic Acids Res | Volume | 24 |
| Issue | 4 | Pages | 694-701 |
| PubMed ID | 8604312 | Mgi Jnum | J:31599 |
| Mgi Id | MGI:79085 | Doi | 10.1093/nar/24.4.694 |
| Citation | Jansa P, et al. (1996) A novel type of retinoic acid response element in the second intron of the mouse H2Kb gene is activated by the RAR/RXR heterodimer. Nucleic Acids Res 24(4):694-701 |
| abstractText | We have identified and characterized a novel retinoic acid (RA) response element (Hi-RARE) in the second intron of the mouse major histocompatibility H2K(b) gene. The Hi- RARE sequence is conserved in all mouse classical and Q class I genes, in MHC class I genes of the rat, Rhesus macaque, cat and in the vast majority of human classical and non-classical class I genes. The Hi-RARE sequence lies within a regulatory element responsible for constitutive expression of a 5' enhancerless H2K(b) gene in the Ltk(-) fibroblasts. Hi-RARE consists of two inverted palindromic RARE consensus sites (5'-PuGGTCA-3') separated by an 8 nt spacer. Mutational analysis revealed that both inverted palindromic hexanucleotide motifs are indispensable functional sites for the 9-cis RA response. The Hi-RARE sequence confers 9-cis RA inducibility to a heterologous promoter. The inducibility is further augmented in embryonal carcinoma cells by the expression of recombinant retinoic acid receptors (RARs) and the retinoid X receptors (RXRs). In vitro, the recombinant RAR/RXR heterodimer creates DNA-protein complex with the Hi-RARE sequence. Treatment of P19 embryonal carcinoma cells with 9C-RA induces the Hi-RARE binding activity of nuclear proteins that proved to be RAR (or RAR-like)/RXR heterodimer. Thus the Hi-RARE represents a new type of RA response element with a role in the modulation of the expression of MHC class I family genes. |
