| First Author | Pisanello F | Year | 2014 |
| Journal | Neuron | Volume | 82 |
| Issue | 6 | Pages | 1245-54 |
| PubMed ID | 24881834 | Mgi Jnum | J:249872 |
| Mgi Id | MGI:6099885 | Doi | 10.1016/j.neuron.2014.04.041 |
| Citation | Pisanello F, et al. (2014) Multipoint-emitting optical fibers for spatially addressable in vivo optogenetics. Neuron 82(6):1245-54 |
| abstractText | Optical stimulation and silencing of neural activity is a powerful technique for elucidating the structure and function of neural circuitry. In most in vivo optogenetic experiments, light is delivered into the brain through a single optical fiber. However, this approach limits illumination to a fixed volume of the brain. Here a focused ion beam is used to pattern multiple light windows on a tapered optical fiber. We show that such fibers allow selective and dynamic illumination of different brain regions along the taper. Site selection is achieved by a simple coupling strategy at the fiber input, and the use of a single tapered waveguide minimizes the implant invasiveness. We demonstrate the effectiveness of this approach for multipoint optical stimulation in the mammalian brain in vivo by coupling the fiber to a microelectrode array and performing simultaneous extracellular recording and stimulation at multiple sites in the mouse striatum and cerebral cortex. |
