| First Author | Crecelius AC | Year | 2008 |
| Journal | Neuroreport | Volume | 19 |
| Issue | 10 | Pages | 1027-31 |
| PubMed ID | 18580573 | Mgi Jnum | J:137870 |
| Mgi Id | MGI:3803077 | Doi | 10.1097/WNR.0b013e3283046157 |
| Citation | Crecelius AC, et al. (2008) The brain proteome profile is highly conserved between Prnp-/- and Prnp+/+ mice. Neuroreport 19(10):1027-31 |
| abstractText | The aim of this study is to compare the proteome of Prnp-/- (Zurich I) gene-ablated mouse brains with wild-type mouse brains. Fluorescence two-dimensional-difference gel electrophoresis (DIGE) and isotope-coded protein labeling (ICPL) were applied for brain homogenates. Similar quantitative protein profiles (<or=1.5-fold change, P<0.05) were obtained by quantitative two-dimensional-DIGE analysis of whole brain homogenates of two developmental stages (days 1 and 67). Complementary ICPL-liquid chromatography-mass spectrometry experiments (n=5) of whole brain homogenates and synaptosomes were performed. The abundance ratios of all proteins quantified did not exceed the statistical spread determined (<2-fold). Therefore, both of the two-dimensional-DIGE and ICPL analyses demonstrated a highly conserved quantitative protein profile in brain homogenates of Prnp-/- and Prnp+/+ mice. |
