| Primary Identifier | MGI:5699848 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Scn1a |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A premature stop codon (CAG to TAG) at amino acid residue E1099 (E1099X) was introduced into the linker between domains 2 and 3. The loxP flanked neo cassette downstream of the mutation was removed via cre-mediated recombination. Western blot analysis indicates that global protein expression is reduced to 54.1% in heterozygotes and is undetectable in homozygotes, indicating a null allele. |
