| Primary Identifier | MGI:3576463 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Sema3b |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 1-6, encoding the first 163 amino acids and ~40% of the SEMA domain, were flanked with loxP sites. An FRT-flanked puromycin resistance cassette was inserted into intron 6. ES cells were transfected with Flp recombinase to remove the puro. RT-PCR demonstrated that the single loxP site in intron 6 did not interfere with splicing between exons 6 and 7. |
