| First Author | Sokoloff D | Year | 1988 |
| Journal | Biochem Genet | Volume | 26 |
| Issue | 9-10 | Pages | 631-44 |
| PubMed ID | 3149468 | Mgi Jnum | J:30943 |
| Mgi Id | MGI:78225 | Doi | 10.1007/BF02399607 |
| Citation | Sokoloff D, et al. (1988) Genetic control of ganglioside biosynthesis in mice. Biochem Genet 26(9-10):631-44 |
| abstractText | The enzymatic basis for the differences in hepatic ganglioside patterns in the mouse strains C57Bl/6 and Swiss White (SW) was investigated. SW has a Swiss-type ganglioside profile, expressing GM1- and GD1a- in addition to GM2- as major hepatic gangliosides, whereas C57Bl/6 shows a GM2-type profile, expressing only GM2- as the major hepatic ganglioside. The enzyme UDP-galactose:GM2 ganglioside galactosyltransferase (GM2-GalT), which catalyzes the synthesis of GM1 ganglioside, showed a four- to fivefold elevation in intact and solubilized liver Golgi membrane fractions of the SW strain compared to C57Bl/6. Crosses between C57Bl/6 and SW produced an F1 generation with a hepatic ganglioside and enzymatic phenotype intermediate between those of the two parental strains. All three genotypic groups show two forms of the Golgi apparatus enzyme with isoelectric points of 6.5-6.8 and 8.3-9.0. The simplest mode of action of genes which control the enzymatic phenotype that would be consistent with these findings are one or two structural genes or one or two cis-regulatory genes affecting the rate of enzyme synthesis. |
