Primary Identifier | MGI:4353890 | Allele Type | Targeted |
Gene | Pou4f2 | Transmission | Germline |
Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Sequence encoding three copies of the hemagglutinin (HA) epitope YPYDVPDYA were fused in frame 3' to the last exon of the gene locus. A floxed neomycin cassette was inserted downstream and was later removed by breeding founders with a cre deleter strain. Mice homozygous for this C-terminal "tagged" allele recapitulated wild-type expression patterns of the endogenous protein. Protein function was unaltered as determined by phenotypic analysis. |