| First Author | Okubo K | Year | 2000 |
| Journal | Gen Comp Endocrinol | Volume | 119 |
| Issue | 2 | Pages | 181-92 |
| PubMed ID | 10936038 | Mgi Jnum | J:64136 |
| Mgi Id | MGI:1888785 | Doi | 10.1006/gcen.2000.7511 |
| Citation | Okubo K, et al. (2000) Molecular cloning and tissue-specific expression of a gonadotropin-releasing hormone receptor in the japanese Eel. Gen Comp Endocrinol 119(2):181-92 |
| abstractText | Gonadotropin-releasing hormone (GnRH) is a key regulatory neuropeptide involved in the control of reproduction in vertebrates. In the Japanese eel, one of the most primitive teleost species, two molecular forms of GnRH, mammalian-type GnRH and chicken-II-type GnRH (cGnRH-II), have been identified. This study has isolated a full-length cDNA for a GnRH receptor from the pituitary of the eel. The 3233-bp cDNA encodes a 380-amino acid protein which contains seven hydrophobic transmembrane domains and N- and C-terminal regions. The exon/intron organization of the open reading frame of the eel GnRH receptor gene was also determined. The open reading frame consists of three exons and two introns. The exon-intron splice site is similar to that of the GnRH receptor genes of mammals reported so far. Expression of the eel GnRH receptor was detected in various parts of the brain, pituitary, eye, olfactory epithelium, and testis. This result suggests that GnRH has local functions in these tissues in addition to its actions on gonadotropin synthesis and release in the pituitary. This tissue-specific expression pattern is similar to that of the eel cGnRH-II. Furthermore, the present eel receptor shows very high amino acid identity with the catfish and goldfish GnRH receptors, which are highly selective for the cGnRH-II. These results suggest that the cGnRH-II acts through binding to the present receptor in the eel. Copyright 2000 Academic Press. |
