| Primary Identifier | MGI:7512765 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inducible, Recombinase, Reporter | Gene | Slc30a3 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting constructs consist of the 3â long homology arm (LA) extends ~6.6 kb 3â to the Rox12/Rox flanked mCherry inversion cassette, and the 5â short homology arm (SA) extends about 3 kb 5â to the 5â Cre/ERT sequence. Homologous recombination in the ES cells inserted neo selection cassette, cre/ERT2 and Rox flanked mCherry into the Slc30a3 gene. The 5â Cre is in-frame fused to the endogenous ATG start site. The 3â CreERT2 is fused with P2A-exon 1 coding sequence. The mCherry inversion cassette is inserted in intron 2. Exon 2 and the inversion cassette are flanked by ROX12/ROX sites for conditionally expressing mCherry reporter. The neo-selection cassette was removed by crossing the resulting chimeric mice to flippase (FLP) deleter mice. |
