| Primary Identifier | MGI:1857765 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Parp1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| description | Null mice appear healthy and fertile with no gross abnormalities. No skin hyperplasia or obesity was observed. These mice were used to examine the role of ADPRP in Streptozotocin induced diabetes. STZ injection caused a significant increase in blood glucose concentrations of wildtype mice as well as necrosis in islet cells leading to an eventual loss on beta cells. In contrast, homozygous mice are protected from STZ-induced diabetes. Glucose levels in these animals remained relatively normal and islet beta cell death was markedly less in these animals (J:53360). |
| molecularNote | A neomycin resistance cassette was inserted into exon 1, 10 bp downstream of the ATG codon. Protein activity was not detected in tissues derived from homozygous mice. Western blot analysis detected a 113 kDa protein in homozygous mice, but this truncated form is predicted to be inactive beacuse it should lack a critical N-terminal zinc finger. |
