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Allele : Col1a1<tm4(CAG-luc2)Nki> collagen, type I, alpha 1; targeted mutation 4, The Netherlands Cancer Institute
Primary Identifier  MGI:5443879 Allele Type  Targeted
Attribute String  Conditional ready, No functional change Gene  Col1a1
Transmission  Germline Strain of Origin  Not Specified
Is Recombinase  false Is Wild Type  false
description  ES cells contained Trp53tm1Brn and Rb1tm2Brn in addition to the targeted Col1a1tm2Nki allele.
molecularNote  FVB;129P2-Trp53 Col1A1 Rb1 were retargeted to replace the frt-flanked PGK-Neo cassette in the 3' UTR of the Col1a1 by a "Flp-in" plasmid encoding a frt-lox66-Cag-lox71-Luc2 inversion transgene, called inv-Cag-Luc2, by co-transfected with a Flpe-expressing plasmid to facilitate recombination into the 3' UTR of the Col1a1 locus. This transgene contains the constitutive CAGGs promoter that drives the expression of the Hygromycin-resistance gene, present in the targeted Col1a1 locus. Upon expression of the Cre recombinase, the promoter is inverted due to flanking mutant loxP sites, i.e. lox66 and lox71, placing the promoter in front of the firefly Luciferase2 gene.
  • mutations:
  • Insertion
  • synonyms:
  • frt-invCag-Luc,
  • Col1A1<tm4(lox66-Cag-lox71-Luc2)Nki>,
  • frt-invCag-Luc,
  • Col1A1<tm4(lox66-Cag-lox71-Luc2)Nki>
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