| First Author | Putkey FR | Year | 2002 |
| Journal | Dev Cell | Volume | 3 |
| Issue | 3 | Pages | 351-65 |
| PubMed ID | 12361599 | Mgi Jnum | J:81046 |
| Mgi Id | MGI:2447982 | Doi | 10.1016/s1534-5807(02)00255-1 |
| Citation | Putkey FR, et al. (2002) Unstable kinetochore-microtubule capture and chromosomal instability following deletion of CENP-E. Dev Cell 3(3):351-65 |
| abstractText | A selective disruption of the mouse CENP-E gene was generated to test how this kinetochore-associated, kinesin-like protein contributes to chromosome segregation. The removal of CENP-E in primary cells produced spindles in which some metaphase chromosomes lay juxtaposed to a spindle pole, despite the absence of microtubules stably bound to their kinetochores. Most CENP-E-free chromosomes moved to the spindle equator, but their kinetochores bound only half the normal number of microtubules. Deletion of CENP-E in embryos led to early developmental arrest. Selective deletion of CENP-E in liver revealed that tissue regeneration after chemical damage was accompanied by aberrant mitoses marked by chromosome missegregation. CENP-E is thus essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. |
