First Author | Fradejas N | Year | 2013 |
Journal | Biochem J | Volume | 450 |
Issue | 2 | Pages | 427-32 |
PubMed ID | 23289710 | Mgi Jnum | J:195090 |
Mgi Id | MGI:5476410 | Doi | 10.1042/BJ20121713 |
Citation | Fradejas N, et al. (2013) Mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression. Biochem J 450(2):427-32 |
abstractText | Selenoproteins are proteins carrying the rare amino acid Sec (selenocysteine). Full expression of selenoproteins requires modification of tRNA([Ser]Sec), including N(6)-isopentenylation of base A(37). We show that Trit1 is a dimethylallyl:tRNA([Ser]Sec) transferase. Knockdown of Trit1 reduces expression of selenoproteins. Incubation of in vitro transcribed tRNA[Ser]Sec with recombinant Trit1 transfers [(14)C]dimethylallyl pyrophosphate to tRNA([Ser]Sec). 37A>G tRNA([Ser]Sec) is resistant to isopentenylation by Trit1. |