| First Author | Zou L | Year | 1995 |
| Journal | Endocrinology | Volume | 136 |
| Issue | 11 | Pages | 5236-9 |
| PubMed ID | 7588264 | Mgi Jnum | J:29764 |
| Mgi Id | MGI:77283 | Doi | 10.1210/endo.136.11.7588264 |
| Citation | Zou L, et al. (1995) A member of the CTF/NF-1 transcription factor family regulates murine growth hormone receptor gene promoter activity. Endocrinology 136(11):5236-9 |
| abstractText | Deletional analysis by transient transfection of COS-7 cells with the murine growth hormone (GH) receptor gene promoter for the L1 transcript identified a 16-bp enhancer element located approximately 3.0 kb upstream of the major transcription start sites. The sequence of this enhancer element suggested a binding motif for the CTF/NF-1 family of transcription factors. In electromobility shift assays (EMSA) this DNA-element formed a sequence-specific complex with nuclear proteins from COS-7 cells. Competition experiments with oligonucleotide containing a consensus binding motif for CTF/NF-1 protein(s) and supershift EMSA with an anti-CTF/NF-1 antibody established that a CTF/NF-1 like protein binds to this enhancer element. |
